Description

Bismark Brown R CAS: 5421-66-9 | MF: C21H24N8•2HCl | MW: 461.39, Buy now Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains. All Tristains products are exclusively distributed by Dawn Scientific Inc.

Bismarck brown R is diazo dye.It appears Brown to Black in color.The chemical formula of Bismarck brown R is C21H26Cl2N8. Its synonyms are C.I. 21010; Basic Brown 4; Vesuvine. Bismark Brown R (BB), also called as basic brown 4, belongs to the class of diazo dyes. Bismarck Brown R is soluble in water. It has the absorption maximum at 468nm. Bismarck Brown R is widely used as a biological stain in histology and microscopy.

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Application :

Bismarck Brown R is used in a modified Coomasie blue staining procedure.
In some cases, it can be used as an indicator, changing color in response to certain chemical conditions.
It is utilized to stain various biological specimens, such as cells, tissues, and microorganisms, to enhance visibility under a microscope. Bismarck Brown R can be used to differentiate cell structures and highlight specific cellular components.

Benefits :

Used as an adsorbate
Used in dye decoloration experiment
Gives reliable results

Additional information

Brand	Tristains
Size	100g, 25g

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TECHNICAL INFORMATION

Alternate Names	5,5-[(4-Methyl-m-phenylene)bis(azo)]bis[toluene-2,4-diamine] dihydrochloride
Application	Bismarck Brown R is a small molecule azo dye
CAS Number	5421-66-9
Molecular Weight	461.39
Molecular Formula:	C₂₁H₂₄N₈•₂HCl

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

Bismarck Brown R is a dye that is not has not been as incorporated as a biological stain as Bismarck Brown Y (sc-214611). The compound has been used in a modified Coomasie blue staining procedure with the effect of Bismarck Brown R reducing the staining and destaining times yet increasing the sensitivity of detection (from 100 ng of BSA to 25 ng).

TECHNICAL INFORMATION

Appearance	Powder
Physical State	Solid
Solubility	Soluble in water, ethanol, acetone (slightly soluble), and cellosolve. Insoluble in benzene.
Storage	Store at room temperature
Boiling Point	729° C at 760 mmHg (Predicted)
Melting Point	222° C (lit.)(dec.)
IC50	the PhoP regulon in Salmonella typhi: IC₅₀= 57.59 µM

SAFETY INFORMATION

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

WGK Germany	3
PubChem CID	79459
MDL Number	MFCD00036386
EC Number	226-541-4
Beilstein Registry	1829673
SMILES	CC1=C(C=C(C=C1)N=NC2=C(C=C(C(=C2)C)N)N)N=NC3=C(C=C(C(=C3)C)N)N.Cl.Cl

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S10034

Bismark Brown R

CAS No

5421-66-9

Synonym(s)

5,5′-[(4-Methyl-m-phenylene)bis(azo)]bis

[toluene-2,4-diamine] dihydrochloride

Molecular Formula:

C₂₁H₂₄N₈•2HCl

Molecular Weight:

461.39

EC Number:

226-541-4

Colour Index Number:

21010

PubChem CID:

79459

MDL number:

MFCD00036386

Specification SDS

Brand	Tristains
Size	100g, 25g

Alternate Names	5,5-[(4-Methyl-m-phenylene)bis(azo)]bis[toluene-2,4-diamine] dihydrochloride
Application	Bismarck Brown R is a small molecule azo dye
CAS Number	5421-66-9
Molecular Weight	461.39
Molecular Formula:	C₂₁H₂₄N₈•₂HCl

Appearance	Powder
Physical State	Solid
Solubility	Soluble in water, ethanol, acetone (slightly soluble), and cellosolve. Insoluble in benzene.
Storage	Store at room temperature
Boiling Point	729° C at 760 mmHg (Predicted)
Melting Point	222° C (lit.)(dec.)
IC50	the PhoP regulon in Salmonella typhi: IC₅₀= 57.59 µM

WGK Germany	3
PubChem CID	79459
MDL Number	MFCD00036386
EC Number	226-541-4
Beilstein Registry	1829673
SMILES	CC1=C(C=C(C=C1)N=NC2=C(C=C(C(=C2)C)N)N)N=NC3=C(C=C(C(=C3)C)N)N.Cl.Cl