Description

Evans Blue C.I. 23860 CAS: 314-13-6 | MF: C34H24N6Na4O14S4 | MW: 960.83, Buy now Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains. All Tristains products are exclusively distributed by Dawn Scientific Inc.

Evans Blue, also known as C.I. 23860 and Direct Blue 53, is a synthetic azo dye with the chemical formula C34H24N6Na4O14S4. It is a dark blue, crystalline powder that dissolves in water and other polar solvents. This dye belongs to the class of diazo dyes and is renowned for its intense, vivid blue color. Evans Blue has a strong affinity for serum albumin, making it an effective high molecular weight protein tracer. This property allows it to be utilized in physiology for estimating the proportion of body water contained in blood plasma. The versatile and valuable nature of Evans Blue has led to its widespread applications in biomedical research, analytical chemistry, and various industrial sectors, taking advantage of its distinctive properties and color characteristics.

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.

Application :

In histology, Evans Blue is used as a staining agent, particularly in combination with other dyes, to enhance the visualization of tissues, cells, and cellular structures under a microscope.
In the field of biomedical research, it is widely used as a tracer dye to study vascular permeability and blood-tissue barrier integrity.
Evans Blue is employed in various protein binding assays, such as albumin-binding studies, due to its ability to bind to serum albumin.

Benefits :

Ensures the longevity of the color in various applications
Versatile and stable
Valuable colorant in various applications where a bold, vibrant blue is desired

Additional information

Synonyms	Direct blue 53; CI 23860
CAS NO	314-13-6
CI No	23860
MW	–
Brand	Tristains
Size	100g, 10g, 25g, 50g

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TECHNICAL INFORMATION

Alternate Names	Direct blue 53; CI 23860
Application	Evans Blue is an inhibitor of EAAT-mediated L-glutamate uptake
CAS Number	314-13-6
Molecular Weight	960.83
Molecular Formula:	C₃₄H₂₄N₆Na₄O₁₄S₄

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

Evans blue is a potent inhibitor of L-glutamate uptake into synaptic vesicles via EAAT. Evans Blue also inhibits AMPA and kainate receptor-mediated currents (IC₅₀ values are 220 and 150 nM respectively). The compound is also a P2X-selective purinoceptor antagonist. Used as a dye, Evans blue reduces unwanted fluorescence due to the conjugation and auto fluorescence of structural elements in the tissue or cells.

TECHNICAL INFORMATION

Appearance	Powder
Physical State	Solid
Solubility	Soluble in water.
Ki Data	Vesicular glutamate transporter 3: K_i= 40 nM (rat); Autotaxin: K_i= 824 nM (human)
Storage	Store at room temperature
pK Values	n²⁰_D ~1.84 (Predicted)
IC50 :	Autotaxin: IC₅₀ = 922 nM (human); Protein-tyrosine phosphatase 1: IC₅₀ = 1200 nM (Saccharomyces cerevisiae); Protein-tyrosine phosphatase 1 B: IC₅₀ = 1300 nM (human)

SAFETY INFORMATION

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

WGK Germany	3
RTECS	QJ6440000
PubChem CID	9566057
Merck Index	14: 3905
MDL Number	MFCD00004021
EC Number	206-242-5
Beilstein Registry :	162259
SMILES :	CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N/N=C3/C(=O)C4=C(C(=CC(=C4N)S(=O)(=O)[O-])S(=O)(=O)[O-])C=C3)C)N/N=C5/C(=O)C6=C(C(=CC(=C6N)S(=O)(=O)[O-])S(=O)(=O)[O-])C=C5.[Na+].[Na+].[Na+].[Na+]