Ponceau S Electrophoresis

Buy Ponceau S Electrophoresis (6226-79-5) MF: C22H12N4Na4O13S4 | MW: 760.57. Get High Quality Ponceau S Electrophoresis (6226-79-5) from TriStains.

as a reversible protein stain for visualizing protein bands on nitrocellulose or PVDF membranes after Western blotting.

“TriStains”, Biological Stains that offer range of stains used in Histology, Cytology, Microbiology and Hematology laboratories.

TriStains meet the highest quality standards and give excellent color performance of desired components of cells and tissue in life science laboratories.

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Applications: Electrophoresis and Protein Transfer: Proteins are separated by gel electrophoresis, typically using polyacrylamide gels, based on their size and charge. After electrophoresis, the proteins are transferred from the gel onto a solid support membrane, such as nitrocellulose or PVDF.Ponceau S Staining: Once the protein transfer is complete, the membrane is briefly immersed in a staining solution containing Ponceau S dye. The dye binds to the proteins, resulting in the development of visible protein bands on the membrane.Destaining: After staining, the excess Ponceau S dye is washed away by rinsing the membrane with a destaining solution or distilled water. The destaining process removes the background stain, leaving only the protein bands visible.Analysis: Following destaining, the protein bands can be visually examined and documented. Ponceau S staining provides a quick and convenient way to assess protein transfer efficiency, confirm the presence of proteins of interest, and estimate their relative molecular weights.