Buy Sudan Black B Certified (4197-25-5) MF: C29H24N6 | MW: 456.54. Get High Quality Sudan Black B Certified (4197-25-5) from TriStains.
Sudan Black B is a synthetic dye that has been used historically in the laboratory setting for various applications.
“TriStains”, Histological Stains/Biological Stains that offer range of stains used in Histology, Cytology, Microbiology and Hematology laboratories.
TriStains meet the highest quality standards and give excellent color performance of desired components of cells and tissue in life science laboratories.
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Applications: Staining agent: Sudan Black B is commonly used as a staining agent in histology and cytology laboratories. It is primarily used to stain lipids, particularly neutral lipids such as triglycerides and lipoproteins. By binding to lipids, Sudan Black B helps visualize lipid-rich structures and lipid droplets within cells.Lipid detection: Sudan Black B staining is often used to detect lipid-containing structures in biological samples. It is commonly employed in research studies involving adipocytes (fat cells), where it can aid in the identification and quantification of lipid content in tissues or cells.Myelin staining: Sudan Black B has also been used to stain myelin in nerve tissue. Myelin is a fatty substance that wraps around nerve fibers and plays a crucial role in facilitating nerve signal transmission. By staining myelin, Sudan Black B can help visualize and study the distribution and integrity of myelin in the nervous system.Compatibility and limitations: Sudan Black B is typically used on frozen or paraffin-embedded tissue sections. It is not compatible with formalin-fixed, paraffin-embedded tissues or with other staining methods that involve deparaffinization. It is important to note that Sudan Black B staining may also interfere with the detection of other cellular components, so it is typically used in combination with other stains for a comprehensive analysis of tissues or cells.Toxicity considerations: While Sudan Black B has been widely used in laboratory research, it is important to handle it with caution. Sudan Black B is considered a potential carcinogen and can be harmful if ingested or inhaled. Proper safety precautions, such as the use of gloves, protective eyewear, and a fume hood, should be followed when working with Sudan Black B.
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