Methylene Blue Alkaline Loffler’S

Methylene Blue Alkaline Loffler’S CAS: 61-73-4 | MF: C16H18ClN3S•xH2O | MW: 319.85, Buy now Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains. All Tristains products are exclusively distributed by Dawn Scientific Inc.

Methylene Blue Alkaline Loffler’s Solution is a staining solution that includes methylene blue dye in an alkaline environment. It is also referred to as Methylene Blue solution, Ehrlich’s reagent III, Loeffler’s Methylene Blue. The chemical formula of this solution is C16H18ClN3S. The solution is commonly made by dissolving methylene blue in a potassium hydroxide (KOH) or sodium hydroxide (NaOH) solution. Methylene Blue Loeffler reagent is utilized for staining acidoresistant bacteria based on Ziehl-Neelsen, utilizing the dye’s ability to stain nearly all the bacterial cell components in a slightly alkaline medium (Methylene Blue Loeffler reagent has a pH value of 8-8.5). Methylene Blue Alkaline Loffler’s Solution is a laboratory reagent employed in microbiology and histology.

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.

Application :

• Methylene Blue Alkaline Loffler’s Solution is commonly used to stain bacterial smears or cultures. It helps in the identification and differentiation of various bacterial species based on their morphological characteristics.
• In histology, this solution can be used to stain cellular structures, such as nuclei, cytoplasm, and certain organelles. It is particularly useful in the examination of tissue sections under a microscope.
• It can be used to specifically stain bacterial spores, which are resistant to certain staining techniques. This helps in the identification and differentiation of spore-forming bacteria.
• It is suitable for staining and monitoring genera such as Gonococcus, Lactobacillus and for visualizing genus Pasteurella. It is also suitable for staining bacterial nucleus and cytoplasm.

Benefits :

• The alkaline nature of the solution enhances the staining properties of methylene blue, allowing for better contrast and visualization of the targeted structures
• Versatile and ease of use
• Ability to selectively stain bacterial spores or differentiate between different bacterial species