Methylene Blue Alkaline Loffler’S

Buy Methylene Blue Alkaline Loffler’S (61-73-4) MF: C16H18ClN3S•xH2O | MW: 319.85. Get High Quality Methylene Blue Alkaline Loffler’S (61-73-4) from Tristains.

Methylene Blue Alkaline Loffler’s Solution is a staining solution that includes methylene blue dye in an alkaline environment. It is also referred to as Methylene Blue solution, Ehrlich’s reagent III, Loeffler’s Methylene Blue. The chemical formula of this solution is C16H18ClN3S. The solution is commonly made by dissolving methylene blue in a potassium hydroxide (KOH) or sodium hydroxide (NaOH) solution. Methylene Blue Loeffler reagent is utilized for staining acidoresistant bacteria based on Ziehl-Neelsen, utilizing the dye’s ability to stain nearly all the bacterial cell components in a slightly alkaline medium (Methylene Blue Loeffler reagent has a pH value of 8-8.5). Methylene Blue Alkaline Loffler’s Solution is a laboratory reagent employed in microbiology and histology.

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Application :

• Methylene Blue Alkaline Loffler’s Solution is commonly used to stain bacterial smears or cultures. It helps in the identification and differentiation of various bacterial species based on their morphological characteristics.
• In histology, this solution can be used to stain cellular structures, such as nuclei, cytoplasm, and certain organelles. It is particularly useful in the examination of tissue sections under a microscope.
• It can be used to specifically stain bacterial spores, which are resistant to certain staining techniques. This helps in the identification and differentiation of spore-forming bacteria.
• It is suitable for staining and monitoring genera such as Gonococcus, Lactobacillus and for visualizing genus Pasteurella. It is also suitable for staining bacterial nucleus and cytoplasm.

Benefits :

• The alkaline nature of the solution enhances the staining properties of methylene blue, allowing for better contrast and visualization of the targeted structures
• Versatile and ease of use
• Ability to selectively stain bacterial spores or differentiate between different bacterial species