Crystal Violet Staining

Buy High Quality Crystal Violet Staining (548-62-9) MF: C25H30ClN3 | MW: 407.98. from Tristains.

Crystal violet, also referred to as gentian violet or methyl violet 10B, is a deep purple dye extensively utilized in the fields of microbiology and histology. This dye, with a chemical formula of C25H30ClN3, is the N-hexamethylated derivative of pararosaniline and belongs to the triphenylmethane family. Renowned for its antibacterial and antifungal properties, crystal violet staining is employed to visualize and distinguish various cellular components in cells or tissues under a microscope. It is a cationic, acidotropic protein dye that is fundamental in histological and bacteriological staining procedures.

TriStains series products are carefully tested to ensure accurate, reliable, and reproducible results. Our products are available in different packaging sizes to allow you to get all types of stains & Indicators for your specific purposes from a single source.

In-addition Tristains also deals in numerous Laboratory Supplies, Chemicals, Equipment, Instruments, Reagents, Standard Solutions, Buffers, Histological Stains/Biological Stains & Indicators and many more, for more information please visit our website www.tristains.com or email to [email protected] we will be happy to help you. All Tristains Products are exclusively distributed by Dawn Scientific Inc (https://dawnscientific.com)

Application :

• Crystal violet staining solution is the primary stain in the Gram staining procedure, a crucial technique in microbiology for classifying bacteria into Gram-positive and Gram-negative groups based on their cell wall structure.
• In histology, it can be used to stain various tissue sections, highlighting cellular structures and aiding in the differentiation of different tissue types.
• It is used in cytology for staining cell smears, providing contrast to cellular components and making it easier to identify and study cells under a microscope.
• Crystal violet staining is used in cell viability assays to assess the metabolic activity and viability of cells. It binds to cellular DNA and proteins, allowing quantification of cell numbers based on dye absorption. It has been successfully used to develop a counterion-staining method to detect DNA in agarose gel electrophoresis.

Benefits :

• Provides strong and clear staining, enhancing the contrast between different cellular components or microorganisms
• Essential for accurately classifying bacteria into Gram-positive and Gram-negative groups
• Produces stable and consistent staining results