Crystal Violet Staining

Crystal Violet Staining CAS: 548-62-9 | MF: C25H30ClN3 | MW: 407.98, Buy now Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains. All Tristains products are exclusively distributed by Dawn Scientific Inc.

Crystal violet, also referred to as gentian violet or methyl violet 10B, is a deep purple dye extensively utilized in the fields of microbiology and histology. This dye, with a chemical formula of C25H30ClN3, is the N-hexamethylated derivative of pararosaniline and belongs to the triphenylmethane family. Renowned for its antibacterial and antifungal properties, crystal violet staining is employed to visualize and distinguish various cellular components in cells or tissues under a microscope. It is a cationic, acidotropic protein dye that is fundamental in histological and bacteriological staining procedures.

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.

Application :

• Crystal violet staining solution is the primary stain in the Gram staining procedure, a crucial technique in microbiology for classifying bacteria into Gram-positive and Gram-negative groups based on their cell wall structure.
• In histology, it can be used to stain various tissue sections, highlighting cellular structures and aiding in the differentiation of different tissue types.
• It is used in cytology for staining cell smears, providing contrast to cellular components and making it easier to identify and study cells under a microscope.
• Crystal violet staining is used in cell viability assays to assess the metabolic activity and viability of cells. It binds to cellular DNA and proteins, allowing quantification of cell numbers based on dye absorption. It has been successfully used to develop a counterion-staining method to detect DNA in agarose gel electrophoresis.

Benefits :

• Provides strong and clear staining, enhancing the contrast between different cellular components or microorganisms
• Essential for accurately classifying bacteria into Gram-positive and Gram-negative groups
• Produces stable and consistent staining results