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Fast Green F.C.F. Certified
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Tristains

S10081

Fast Green F.C.F. Certified

CAS No
2353-45-9
Synonym(s)
Solid Green FCF; Food Green No. 3
Molecular Formula:
C37H34N2Na2O10S3
Molecular Weight:
808.85
EC Number:
219-091-5
Colour Index Number:
42053
PubChem CID:
16887
MDL number:
MFCD00013053
Specification SDS Available pack size: 10g, 25g, 50g, 100g, 5g
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Description

Fast Green F.C.F. Certified CAS: 2353-45-9 | MF: C37H34N2O10S3Na2 | MW: 808.85, Buy now for reliable and reproducible results from Tristains.

Fast Green F.C.F. Certified C.I. 42053 or Food Green 3 (CAS No. 2353-45-9) is a triarylmethane dye that is a synthetic, water-soluble, triarylmethane dye commonly used in both laboratory and analytical procedures. It is a dark green crystalline powder, which is extremely stained and is often used as an additive food colorant and a laboratory stain. Fast Green FCF has a chemical structure C37H34N2O10S3Na2, and therefore is highly applicable in a biological and chemical staining method. Fast Green FCF Certified can be used in the staining of plant cells in safranin-fast green staining; it is one of the effective substitutes to aniline blue in the one-step trichrome staining protocol suggested by Gomori. It is very sensitive to detect proteins in polyacrylamide gels, and specifically during isoelectric focusing. Also, this dye is commonly employed as a cytological counterstain and a mammalian tissue stain of collagen and muscle fiber.

Applications

  • Histology and Cytology Staining: Fast Green FCF Certified is widely used in histology where it can be used in conjunction with Safranin to stain plant tissue. Safranin-fast green procedure improves the staining of plant tissue structure and cell morphology. It can also be used to stain collagen deposits, proteins and starch, which greatly enhances tissue contrast when seen under microscopes.
  • Gomori’s One-Step Trichrome Staining: Fast Green FCF Certified is frequently used in histopathology as a substitute to aniline blue in the Gomori one-step trichrome technique. This technique is necessary in the separation of muscle fibers and collagen and connective tissues and the green stain allows easy differentiation of tissues.
  • Protein Staining in Electrophoresis: Fast Green FCF Certified is a dependable protein stain applied in isoelectric focusing (IEF), native PAGE, and SDS-PAGE to present sensitive and reproducible visualization of proteins.
  • Chromatography and Spectrophotometry: The dye can be used as a tracer or indicator in chromatographic and spectrophotometric analysis. Its green color is stable and bright, and this allows the proper and accurate detection in all sorts of chemical and biological experiments.
  • Cytological Counterstaining: Fast Green FCF Certified is an alternative counterstain in cytology to provide more contrast and clarity of cellularstructures to enable a better microscopic examination.

Key features

  • Certified Coloring Agent: Fast Green FCF Certified is produced in accordance with the standards of certified quality assurance in terms of safety, reliability, and applicability in sciences and industry.
  • High-Intensity and Stable Color: This dye provides steady and bright green stain with good stability, which makes it the right choice in routine and advanced staining processes.
  • Versatile Laboratory Dye: Fast Green FCF is a versatile staining reagent due to its wide range of applications in histology, cytology, electrophoresis, chromatography, and analytical chemistry.

General Description

Fast Green F.C.F. Certified (CAS No. 2353-45-9) is a synthetic, water-soluble dye and is of the triarylmethane group of colorants. It is highly stained and has a strong dark green color that has made it have extensive applications in histology, cytology, microbiology, and analytical chemistry. Having a molecular formula of C37H34N2O10S3Na2 and a molecular weight of 808.85, this dye is very dependable in the accuracy of staining and better visualization during laboratory processes.

If you are looking for where to buy Fast Green F.C.F. Certified (CAS No. 2353-45-9), TriStains offers a wide selection of laboratory research, diagnostic, and industrial histological stain and dye products. Technical specifications of Fast Green F.C.F. Certified are provided in full in order to make informed choices in purchase. In terms of pricing and bulk quantity, customers are also advised to directly contact the company to get the right and competitive information. Order Fast Green F.C.F. Certified from TriStains for reliable and reproducible results.

About Brand

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.

Additional information
Brand

Tristains

Size

100g, 10g, 25g, 50g, 5g

CAS No

2353-45-9

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TECHNICAL INFORMATION
Alternate Names Solid Green FCF; Food Green No. 3
Application Fast Green FCF is a widely utilized tissue and protein stain used in IEF & SDS-PAGE, fluoresces near IR
CAS Number 2353-45-9
Molecular Weight 808.85
Molecular Formula C37H34N2O10S3Na2

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

Fast Green FCF has been a widely utilized tissue and protein stain used in IEF and SDS-PAGE. Has the ability to determine efficiency of transfer and localization of sample proteins on blotting membranes. Other applications of Fast Green FCF include using its clastogenic properties and it may also act at a presynaptic locus inhibiting neurotransmitter release in whole-cell voltage clamped hippocampal interneurons. Has been shown to be a beneficial stain to detail integumental injuries in Chinook salmon. When bound to protein, Fast Green FCF fluoresces near infrared; color index #42053.

TECHNICAL INFORMATION

Appearance Powder or granules
Physical State Solid
Solubility Soluble in water (150 g/l) at 25° C, glycerin, glycol, alcohol, and ethanol.
Storage Store at room temperature
Melting Point 290° C (lit.)(dec.)

SAFETY INFORMATION

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany 2
RTECS BQ4425000
PubChem CID 16887
Merck Index 14:3941
MDL Number MFCD00013053
EC Number 219-091-5
Beilstein Registry 5718212
SMILES CCN(CC1=CC(=CC=C1)S(=O)(=O)[O-])C2=CC=C(C=C2)C(=C3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3)C5=C(C=C(C=C5)O)S(=O)(=O)[O-].[Na+].[Na+]

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