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Tristains

S10095

Giemsa Stain Certified

$95.42 – $259.42

CAS No
51811-82-6
Synonym(s)
Giemsa Stain
Molecular Formula:
C14H14ClN3S
Molecular Weight:
291.8
EC Number:
–
Colour Index Number:
42555
PubChem CID:
13735
MDL number:
MFCD00081642
Specification SDS

Available Options:

Description

Buy Giemsa Stain Certified (51811-82-6) MF: C14H14ClN3S | MW: 291.8. Get High Quality Giemsa Stain Certified (51811-82-6) from Tristains.

Giemsa Stain Certified displays a color ranging from dark green to very dark green. Its chemical formula is C14H14ClN3S and it is also known as Azure mixture sicc. Giemsa stain is a versatile polychromatic stain suitable for staining a wide variety of specimens. It is a buffered thiazine-eosinate solution designed to provide coloration of blood cells. The staining solution is composed of methylene blue, azure, and eosin. It is certified for Giemsa stain for blood films and for the Wolbach′s Giemsa method for paraffin sections. Giemsa Stain Certified is a high-quality stain commonly used in cytogenetics, histology, and parasitology.

“TriStains”, Histological Stains/Biological Stains that offer range of stains used in Histology, Cytology, Microbiology and Hematology laboratories. TriStains meet the highest quality standards and give excellent color performance of desired components of cells and tissue in life science laboratories. TriStains series products are carefully tested to ensure accurate, reliable, and reproducible results. Our products are available in different packaging sizes to allow you to get all types of stains & Indicators for your specific purposes from a single source.

In-addition Tristains also deals in numerous Laboratory Supplies, Chemicals, Equipment, Instruments, Reagents, Standard Solutions, Buffers, Histological Stains/Biological Stains & Indicators and many more, for more information please visit our website www.tristains.com or email to [email protected] we will be happy to help you. All Tristains Products are exclusively distributed by Dawn Scientific Inc (https://dawnscientific.com)

Application :

  • Giemsa’s stain is frequently used for diagnostic purposes in hematology to differentiate the nuclear and cytoplasmic morphology of platelets, RBCs, WBCs, and parasites.
  • It is frequently used in combination with other dye solutions as May-Grunwald’s solution for Pappenheim (MGG) and Wright-Giemsa.
  • The Giemsa stain was adapted to histology due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements.
  • Giemsa stain has been used for the staining of cells and blood films. It is required for the staining of azurophil granules and is an excellent stain for inclusion bodies.
  • Its applications can be seen in hematology for blood and bone marrow specimens, bacteriology, clinical cytology specimens, histological biopsies, and tumor samples.

Benefits :

  • Ensures that the stain maintains a high level of purity and consistency
  • Produces consistent and reliable staining results
  • Versatile and effective
Additional information
Weight N/A
Dimensions N/A
Brand

Size

25g, 5g
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TECHNICAL INFORMATION

Giemsa Stain is a laboratory reagent and stain for microscopy. The stain functions by rearranging chromatin and inducing G bands producing a high quality stain for chromatin and the nuclear membrane. Giemsa Stains have been used for the histopathologic detection of malaria and other parasites such as spirochete and protozoan. The stain has also been incorporated in differential staining between bacterial and human cells. The compound has been used in combination with May-Grunwald or Wright Stain (sc-203763) as a histological hematology stain. However, the May-Grunwald-Giemsa procedure produces a more intense coloration than Wright-Giemsa.

Alternate Names Giemsa solution
Application Giemsa Stain is a high quality stain for chromatin and the nuclear membrane
CAS Number 51811-82-6
Molecular Weight 291.8
Molecular Formula C14H14ClN3S

SAFETY INFORMATION

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID 13735
MDL Number MFCD00081642
EC Number 257-438-2
SMILES C[N+](=C1C=CC2=NC3=C(C=C(C=C3)N)SC2=C1)C.[Cl-]

TECHNICAL INFORMATION

Usage : Technique used to uniformly stain chromosomes that will leave centromeres constricted:

Method 1
materials:
4 mL Giemsa; 96 mL pH 6.8 phosphate buffer

procedure:
1.Place slides in a Coplin jar or staining dish.
2.Prepare the working stain and pour it over the slides.
Stain for 7 minutes.
3.Rinse slides in two changes of distilled water.
4.Air dry slides; mount them with a cover slip if desired. (If sequential banding procedures are to follow, cover slipping is not recommended.)

Method 2
materials:
1.5N HCl
2.Distilled water
3.pH 6.8 phosphate buffer
4. Giemsa stain: 2 mL stock Giemsa; 4 mL pH 6.8 buffer; 92 mL distilled water

procedure:
1.Place slides in a Coplin jar containing 5N HCl for 10 minutes at room temperature.
2.Rinse with tap water for 10 minutes.
3.Stain for in Giemsa for 10 minutes.
4.Rinse, dry, and coverslip, if desired.
Physical State Solid
Solubility insoluble in water (<0.1%), methanol (5.8 mg/l), and ethanol.
Storage Store at room temperature
Melting Point 300° C (lit.)

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