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Giemsa Stain Certified
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Tristains

S10095

Giemsa Stain Certified

CAS No
51811-82-6
Synonym(s)
Giemsa Stain
Molecular Formula:
C14H14ClN3S
Molecular Weight:
291.8
EC Number:
–
Colour Index Number:
42555
PubChem CID:
13735
MDL number:
MFCD00081642
Specification SDS Available pack size: 5g, 25g
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Description

Giemsa Stain Certified CAS: 51811-82-6 | MF: C14H14ClN3S | MW: 291.8, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains.

Giemsa Stain Certified is a dark green to black colored powder that is also referred to as Azure mixture sicc. It is C14H14ClN3S having a molecular weight of 291.8. This polychromic stain is largely employed in the staining of a wide variety of biological specimens. It is a buffered thiazine-eosinate solution that is developed to give clear colouration of blood cells. The staining solution is comprised of eosin, azure dyes and methylene blue. Giemsa Stain Certified can be used to stain blood films and on the Wolbach method of Giemsa staining on paraffin sections. It finds wide application in cytogenetics, histology and parasitology due to its accuracy and ability to stain effectively.

Applications

  • Giemsa Stain Certified is frequently applied in the field of hematology diagnostics to distinguish between nuclear and cytoplasmic morphology of platelets, red blood cells (RBCs), white blood cells (WBCs), and parasites.
  • It is commonly used in combination with other dye solutions like May-Grunwald stain in Pappenheim (MGG) and Wright-Giemsa staining methods.
  • It is commonly used in the staining of cells and blood films, especially in the staining of azurophilic granules and inclusion bodies.
  • It is applicable in hematology when using blood and bone marrow samples, in bacteriology, cytology, and histology.

Key features

  • Multipurpose and Reliable: Most suitable in case of hematology, parasitology, cytology, and histology.
  • Consistent Coloration: Provides uniform staining with low levels of background interference, providing high-clarity microscopic data.
  • Cost-Effective Solution: Proposed good quality work at a low cost that faces all types of laboratories.

General Description

The TriStains Giemsa Stain Certified is a reliable stain that has been known to give a high degree of reliability and reproducibility in the staining procedures within the United States. In use with blood films, bone marrow smears or chromosomal analysis, this certified Giemsa stain provides high-contrast and detailed visualization necessary to make accurate diagnostic interpretations and scientific studies.

Want to buy Giemsa Stain Certified? Buy from TriStains, a reputable supplier of Histological, Cytological, Microbiological, and Hematological dyes and stains. In several different sizes of packaging, in reliable quality, with quick delivery. This certified Giemsa stain comes in many packaging sizes and it is produced according to high standards in the laboratory. Every batch is quality checked so as to provide reliable results in all applications. Labs are able to call the sales office to receive new prices, technical specifications, and bulk purchasing. Order now for reliable and reproducible results from TriStains.

About Brand

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.

Additional information
Brand

Tristains

Size

25g, 5g

CAS No

51811-82-6

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TECHNICAL INFORMATION

Giemsa Stain is a laboratory reagent and stain for microscopy. The stain functions by rearranging chromatin and inducing G bands producing a high quality stain for chromatin and the nuclear membrane. Giemsa Stains have been used for the histopathologic detection of malaria and other parasites such as spirochete and protozoan. The stain has also been incorporated in differential staining between bacterial and human cells. The compound has been used in combination with May-Grunwald or Wright Stain (sc-203763) as a histological hematology stain. However, the May-Grunwald-Giemsa procedure produces a more intense coloration than Wright-Giemsa.

Alternate Names Giemsa solution
Application Giemsa Stain is a high quality stain for chromatin and the nuclear membrane
CAS Number 51811-82-6
Molecular Weight 291.8
Molecular Formula C14H14ClN3S

SAFETY INFORMATION

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID 13735
MDL Number MFCD00081642
EC Number 257-438-2
SMILES C[N+](=C1C=CC2=NC3=C(C=C(C=C3)N)SC2=C1)C.[Cl-]

TECHNICAL INFORMATION

Usage : Technique used to uniformly stain chromosomes that will leave centromeres constricted:

Method 1
materials:
4 mL Giemsa; 96 mL pH 6.8 phosphate buffer

procedure:
1.Place slides in a Coplin jar or staining dish.
2.Prepare the working stain and pour it over the slides.
Stain for 7 minutes.
3.Rinse slides in two changes of distilled water.
4.Air dry slides; mount them with a cover slip if desired. (If sequential banding procedures are to follow, cover slipping is not recommended.)

Method 2
materials:
1.5N HCl
2.Distilled water
3.pH 6.8 phosphate buffer
4. Giemsa stain: 2 mL stock Giemsa; 4 mL pH 6.8 buffer; 92 mL distilled water

procedure:
1.Place slides in a Coplin jar containing 5N HCl for 10 minutes at room temperature.
2.Rinse with tap water for 10 minutes.
3.Stain for in Giemsa for 10 minutes.
4.Rinse, dry, and coverslip, if desired.

Physical State Solid
Solubility insoluble in water (<0.1%), methanol (5.8 mg/l), and ethanol.
Storage Store at room temperature
Melting Point 300° C (lit.)

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