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Tristains

S10117

Methylene Blue Certified

CAS No
7220-79-3
Synonym(s)
C.I. Basic Blue 9, trihydrate (8CI); Methylthionine chloride
Molecular Formula:
C16H18N3S•Cl•3H2O
Molecular Weight:
373.9
EC Number:
200-515-2
Colour Index Number:
–
PubChem CID:
104827
MDL number:
MFCD00012111
Specification SDS
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Description

Methylene Blue Certified CAS: 7220-79-3 | MF: C16H18N3S•Cl•3H2O | MW: 373.9, Buy now Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains. All Tristains products are exclusively distributed by Dawn Scientific Inc.

Methylene blue is a compound that presents itself as either crystals or crystalline powder, with a shine similar to bronze. Its chemical formula is C16H24ClN3O3S. It can dissolve in water and chloroform, and is partially soluble in alcohol. It is also referred to as Basic Blue 9, Tetramethylthionine chloride. This photoactive dye belongs to the phenothiazine family and is commonly utilized in histology, bacteriology (staining), compounding of blood stains, and has a wide range of applications in various industries.

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.

Application :

  • Methylene blue certified has been used to stain cells, and to evaluate cell survival by staining.
  • It mediates the inactivation of enveloped viruses in plasma, when exposed to visible light.

Benefits :

  • Ensures consistent purity, potency, and compliance with industry standards
  • Versatile and stable
Additional information
Brand

Tristains

Size

100g, 10g, 25g, 50g, 5g

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TECHNICAL INFORMATION
Alternate Names C.I. Basic Blue 9, trihydrate (8CI); Methylthionine chloride
Application Methylene blue trihydrate is an inhibitor of cGMP-mediated processes and guanylyl cyclase, a useful stain, and a redox indicator
CAS Number 7220-79-3
Molecular Weight 373.90
Molecular Formula: C16H18N3S•Cl•3H2O

Methylene Blue acts as a GCS (guanylyl cyclase) inhibitor, biological stain and redox indicator. Methylene Blue inhibits tau filament formation. Additionally, Methylene Blue is also reported to inhibit NOSs (nitric oxide synthase). Methylene Blue is widely used to inhibit cGMP-mediated processes. Methylene Blue has been observed to dose-dependently induce cytotoxicity in U-373 MG human astrocytoma and SK-N-MC human neuroblastoma cells. Furthermore Methylene Blue is reported to bind to the M2 subtype of muscarinic receptors in rat cardiac myocytes, and thus exhibit antimuscarinic effects on IK, ACh and ICa. Methylene Blue has also been reported to inhibit endothelium-dependent relaxation of isolated blood vessels.

TECHNICAL INFORMATION

Appearance Crystalline powder
Physical State Solid
Solubility Soluble in water (50 mg/ml), ethanol (70 mg/ml), alcohol, and chloroform. Insoluble in ether.
Storage Store at room temperature
Melting Point 190° C
IC50 : Plasmodium falciparum: IC50 = 5.4 nM; Glutathione reductase: IC50 = 6.4 µM (Plasmodium falciparum 3D7); Apaf-1: IC50 = 15.5 µM (human); Glutathione reductase: IC50 = 16 µM (human)
pK Values : pKb: 4.89 (Predicted)

SAFETY INFORMATION

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany 3
RTECS SP5740000
PubChem CID 104827
Merck Index 14:6060
MDL Number MFCD00150008
EC Number 200-515-2
Beilstein Registry 3599847
SMILES : CN(C)C1=CC2=C(C=C1)N=C3C=CC(=[N+](C)C)C=C3S2.O.O.O.[Cl-]

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