Tristains products are exclusively distributed by Dawn Scientific | Please visit www.dawnscientific.com to place your order.
  • Find SDS & COA
  • APPLICATION
    • Hematology Stains
    • Staining – Histology / Cytology
    • Eosins & Hematoxylins
Tristains products are exclusively distributed by Dawn Scientific | Please visit www.dawnscientific.com to place your order.
TriStains logo for laboratory chemical solutions, ready-to-use stains, and solvents for research and testing
  • Quick Reagents
  • Histological stains
    • Dry Stains & Dyes
    • Stain Solution
  • Indicators
    • Indicator Solid
    • Indicator Solution
  • Solutions
  • Solvents
  • Contact us
  • Become Distributor
Menu
TriStains logo for laboratory chemical solutions, ready-to-use stains, and solvents for research and testing
Methylene Blue Certified
Click to enlarge
Tristains

S10117

Methylene Blue Certified

CAS No
7220-79-3
Synonym(s)
C.I. Basic Blue 9, trihydrate (8CI); Methylthionine chloride
Molecular Formula:
C16H18N3S•Cl•3H2O
Molecular Weight:
373.9
EC Number:
200-515-2
Colour Index Number:
–
PubChem CID:
104827
MDL number:
MFCD00012111
Specification SDS Available pack size: 10g, 25g, 50g, 100g, 5g
Share:
  • Description
  • Additional information
  • Reviews (0)
  • TECHNICAL INFORMATION
Description

Methylene Blue Certified CAS: 7220-79-3 | MF: C16H18N3S•Cl•3H2O | MW: 373.9, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains.

The Methylene Blue Certified is packaged as small crystals or crystalline powder of a typical bronze-like luster. Its chemical structure is expressed in the following molecular formula: C16H24ClN3O3S. The compound can easily dissolve in water and chloroform with some solubility in alcohol. Photoactive dyes are commonly referred to as Basic Blue 9 or Tetramethylthionine Chloride and are a class of phenothiazine based compounds. It finds wide application in the staining of histological materials, bacteriological practices, in the preparation of blood stains and in the most diverse laboratory and industrial implementations.

Applications

  • Applicable to cellular stain and measure cell viability and cell survival.
  • Applied in the titration of analyses of redox indicators in analytical chemistry.

Key features

  • High Quality: Gives sharp, vivid, and crisp staining with the ability to produce results that can be analysed and studied under the microscope.
  • Stable and Reliable: Is highly chemically stable with a good shelf life in a normal laboratory storage environment.
  • Versatile Application: Applies effectively as a biologic stain, chemical marker, or reagent in a variety of testing platforms.
  • Cost-Effective: Available at competitive prices, high-grade Methylene Blue Certified is made available to laboratories in the United States.

General Description

Methylene Blue Certified (CAS No. 61-73-4, MF: C16H24ClN3O3S MW: 373.90) is a reliable and common dye that is used in histological and analytical studies. This certified-grade reagent was supplied as part of the TriStains product line and guarantees the consistency, integrity, and reliability of results across batch-to-batch and among batches. Its excellent staining capacity and chemical stability make it a good tool to be used in cellular analysis, bacteriology differentiation, and oxidation-reduction research in science labs.

Want to buy Methylene Blue Certified? TriStains can be relied upon to supply Methylene Blue Certified to laboratories interested in buying Methylene Blue Certified in the USA with the assurance of purity and full technical documentation. Competitive pricing helps in the requirements of research institutes, universities, and diagnostic laboratories. By using this certified product, the staining clarity is very good, which is proven to be pure and reliable in laboratory performance and thus is a good choice among the scientific community. Select the Methylene Blue Certified offered by TriStains as one that gives better staining clarity, purity is proven, and stable laboratory behavior. Having certified quality and cost-effective solutions, it will be the solution of choice for professionals in the scientific community.

About Brand

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.

Additional information
Brand

Tristains

Size

100g, 10g, 25g, 50g, 5g

CAS No

7220-79-3

Reviews (0)

Reviews

There are no reviews yet.

Be the first to review “Methylene Blue Certified” Cancel reply

Your email address will not be published. Required fields are marked *


The reCAPTCHA verification period has expired. Please reload the page.

TECHNICAL INFORMATION
Alternate Names C.I. Basic Blue 9, trihydrate (8CI); Methylthionine chloride
Application Methylene blue trihydrate is an inhibitor of cGMP-mediated processes and guanylyl cyclase, a useful stain, and a redox indicator
CAS Number 7220-79-3
Molecular Weight 373.90
Molecular Formula: C16H18N3S•Cl•3H2O

Methylene Blue acts as a GCS (guanylyl cyclase) inhibitor, biological stain and redox indicator. Methylene Blue inhibits tau filament formation. Additionally, Methylene Blue is also reported to inhibit NOSs (nitric oxide synthase). Methylene Blue is widely used to inhibit cGMP-mediated processes. Methylene Blue has been observed to dose-dependently induce cytotoxicity in U-373 MG human astrocytoma and SK-N-MC human neuroblastoma cells. Furthermore Methylene Blue is reported to bind to the M2 subtype of muscarinic receptors in rat cardiac myocytes, and thus exhibit antimuscarinic effects on IK, ACh and ICa. Methylene Blue has also been reported to inhibit endothelium-dependent relaxation of isolated blood vessels.

TECHNICAL INFORMATION

Appearance Crystalline powder
Physical State Solid
Solubility Soluble in water (50 mg/ml), ethanol (70 mg/ml), alcohol, and chloroform. Insoluble in ether.
Storage Store at room temperature
Melting Point 190° C
IC50 : Plasmodium falciparum: IC50 = 5.4 nM; Glutathione reductase: IC50 = 6.4 µM (Plasmodium falciparum 3D7); Apaf-1: IC50 = 15.5 µM (human); Glutathione reductase: IC50 = 16 µM (human)
pK Values : pKb: 4.89 (Predicted)

SAFETY INFORMATION

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany 3
RTECS SP5740000
PubChem CID 104827
Merck Index 14:6060
MDL Number MFCD00150008
EC Number 200-515-2
Beilstein Registry 3599847
SMILES : CN(C)C1=CC2=C(C=C1)N=C3C=CC(=[N+](C)C)C=C3S2.O.O.O.[Cl-]

Related products

Fast Green F.C.F. Certified

Fast Green F.C.F. Certified

Catalog: S10081
Buy Now
Eriochrome Black T ACS

Eriochrome Black T ACS

Catalog: S10070
Buy Now
Malachite Green Oxalate

Malachite Green Oxalate

Catalog: S10111
Buy Now
Acid Blue 29

Acid Blue 29

Catalog: S10002
Buy Now
Eosin B Spirit Soluble

Eosin B Spirit Soluble

Catalog: S10067
Buy Now
Saffron C.I.

Saffron C.I.

Catalog: S10173
Buy Now
Safranine O Certified

Safranine O Certified

Catalog: S10174
Buy Now
Sudan III Certified

Sudan III Certified

Catalog: S10179
Buy Now
TriStains logo for laboratory chemical solutions, ready-to-use stains, and solvents for research and testing
ABOUT
  • About Us
  • Contact Us
  • Privacy Policy
  • Terms & Conditions
RESOURCES
  • Useful Links
ACCREDITATION
  • ISO 9001:2015

© 2026 Tristains. All rights reserved

  • Quick Reagents
  • Histological stains
    • Dry Stains & Dyes
    • Stain Solution
  • Indicators
    • Indicator Solid
    • Indicator Solution
  • Solutions
  • Solvents
  • Contact us
  • Become Distributor
Categories
  • Scientific Articles
Recent Posts
  • S10097_webimg
    Hematoxylin
    April 4, 2024 No Comments
  • cytology-2
    Study Of Cytology
    March 1, 2024 No Comments
  • webimg-blog
    4,5- Dibromo fluorescein
    February 16, 2024 No Comments
OUR INSTAGRAM
8769 795
3172 846
4182 642
6413 583
5250 9
3887 491
7167 321
7226 828
5194 40
Recent Comments
    March 2026
    M T W T F S S
     1
    2345678
    9101112131415
    16171819202122
    23242526272829
    3031  
    « Apr    
    Categories
    • Scientific Articles
    March 2026
    M T W T F S S
     1
    2345678
    9101112131415
    16171819202122
    23242526272829
    3031  
    « Apr    
    Facebook Instagram linkedin