Description

Nile Red CAS: 7385-67-3 | MF: C20H18N2O2 | MW: 318.37, Buy now Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains. All Tristains products are exclusively distributed by Dawn Scientific Inc.

Nile Red, also known as Nile Blue Oxazone, is a greenish red crystalline powder with the chemical formula C20H18N2O2. It is a lipophilic stain that is used to stain intracellular lipid droplets yellow. This fluorescent dye has various applications, especially in the fields of biological and analytical chemistry. Its chemical name is 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one.

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.

Application :

Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells.
It has applications in cell biology, where it can be used as a membrane dye which can be readily visualized using an epifluorescence microscope with excitation and emission wavelengths usually shared with red fluorescent protein.

Benefits :

Exhibits a strong fluorescence signal when in a hydrophobic environment
Versatile and cost-effective

Additional information

CAS NO	7385-67-3
Synonyms	Nile Red is also known as 9-(diethylamino)-5H-benzo[a]phenoxazin-5-one and 9-(diethylamino)benzo[a]phenoxazin-5-one
CI No	–
MW	–
Brand	Tristains
Size	100g, 10g, 25g, 50g, 5g

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TECHNICAL INFORMATION

Alternate Names	Nile Red is also known as 9-(diethylamino)-5H-benzo[a]phenoxazin-5-one and 9-(diethylamino)benzo[a]phenoxazin-5-one.
Application	Nile Red is a photostable, lipophilic stain with bright red fluorescence that has been used stain cell structures to visualize and localize colloidal dru
CAS Number	7385-67-3
Molecular Weight	318.37
Molecular Formula:	C₂₀H₁₈N₂O₂

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

Nile Red is an uncharged, hydrophobic, photostable molecule that strongly fluoresces bright red in hydrophobic environments. Nile Red is a lipophilic stain commonly used for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. Nile Red has been shown to detect the exposure or formation of new hydrophobic surfaces induced by ligand binding to calmodulin, oligomerization of melittin, or unfolding of ovalbumin during early thermal denaturation. Nile Red has been employed to stain cell structures, as well as to visualize and localize colloidal drug carriers. Nile Red s fluorescence is unaffected between pH 4.5 and 8.5 and may be used in dilute concentrations to investigate hydrophobic protein sites.

TECHNICAL INFORMATION

Appearance	Powder
Physical State	Solid
Solubility	Soluble in water (Partly miscible), and methanol (1 mg/ml).
Storage	Store at room temperature
Melting Point	203-205° C (lit.)
Boiling Point	484.70° C at 760 mmHg (Predicted)
Density	~1.2 g/cm3 (Predicted)
Refractive Index	n20D 1.64 (Predicted)

SAFETY INFORMATION

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

WGK Germany	3
PubChem CID	65182
Merck Index	14: 10229
MDL Number	MFCD00011639
EC Number	230-966-0
Beilstein Registry	279110
SMILES	CCN(CC)C1=CC2=C(C=C1)N=C3C4=CC=CC=C4C(=O)C=C3O2