Rhodamine B

Buy High Quality Rhodamine B (81-88-9) MF: C28H31N2O3•Cl | MW: 479.01. from Tristains.

Rhodamine B is a useful fluorochrome for histology due to its fluorescent chemical properties in ultraviolet light. It ejects an electron onto TiO2, that’s then scavenged through O2 forming the O2- radical anion. This anion in the end turns into the OH radical. A pH-impartial process degrades Rhodamine B in TiO2 dispersions containing no dodecylbenzenesulfonate. The compound has been included in a FRET system based on lanthanide-doped nanoparticles for tracking biological consequences. Additionally , Rhodamine B has been used as a mitochondrial probe to study the active enegetic of multi-drug resistant and sensitive cells where the mitochondrial Rhodamine B concentration become precisely determined with the lack of fluorescence in the presence of MTT Formazan (sc-215399), a fluorescence quencher. Further, Rhodamine B has been observed to noticeably reduce the number of human lip fibroblast cells in culture. Used for staining Negri bodies in tissue.

“TriStains”, Histological Stains/Biological Stains that offer range of stains used in Histology, Cytology, Microbiology and Hematology laboratories.

TriStains meet the highest quality standards and give excellent color performance of desired components of cells and tissue in life science laboratories.

TriStains series products are carefully tested to ensure accurate, reliable, and reproducible results. Our products are available in different packaging sizes to allow you to get all types of stains & Indicators for your specific purposes from a single source.

In-addition Tristains also deals in numerous Laboratory Supplies, Chemicals, Equipment, Instruments, Reagents, Standard Solutions, Buffers, Histological Stains/Biological Stains & Indicators and many more, for more information please visit our website www.tristains.com or email to [email protected] we will be happy to help you. All Tristains Products are exclusively distributed by Dawn Scientific Inc (https://dawnscientific.com)

Applications: Fluorescent Microscopy: Rhodamine B is widely used in fluorescence microscopy to label and visualize various cellular structures and components. It can be conjugated to antibodies or other biomolecules to target specific proteins or cellular markers of interest. Rhodamine B emits a red-orange fluorescence when excited by appropriate wavelengths of light, allowing for the visualization and analysis of labeled specimens.Cell Viability Assays: Rhodamine B is employed in cell viability assays, such as the rhodamine B assay or the Rhodamine B Lactate Dehydrogenase (LDH) Cytotoxicity Assay. These assays measure cellular metabolic activity and cytotoxicity by assessing the uptake or release of Rhodamine B dye by viable or damaged cells. The dye is taken up by live cells and accumulates in their mitochondria, producing fluorescence.pH Indicator: Rhodamine B can function as a pH indicator in laboratory applications. It exhibits a color change depending on the pH of the solution. It appears yellow in acidic conditions and turns red or pink in alkaline environments. This property makes it useful for monitoring and measuring pH changes in various assays and experimental setups.Protein and Nucleic Acid Staining: Rhodamine B can be used to stain proteins and nucleic acids. It can bind to nucleic acids, such as DNA or RNA, and visualize their presence in gel electrophoresis or other separation techniques. Additionally, Rhodamine B can be utilized for protein gel staining to visualize proteins in polyacrylamide gels.Flow Cytometry: Rhodamine B is used as a fluorescent dye in flow cytometry, a technique for analyzing and sorting individual cells based on their physical and fluorescence properties. Rhodamine B-labeled antibodies or other fluorophores are used to label specific cell surface markers or intracellular components, enabling their identification and characterization.