Description

Acridine Orange Base CAS: 494-38-2 | MF: C17H19N3 | MW: 265.35, Buy now Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains. All Tristains products are exclusively distributed by Dawn Scientific Inc.

Acridine Orange Base, a fluorescent dye, has several applications in biological and biomedical research. A member of the class of aminoacridines that is acridine carrying two dimethylamino substituents at positions 3 and 6. The hydrochloride salt is the fluorescent dye acridine orange, used for cell cycle determination. It is also known as Solvent Orange 15. Its stability at low pH allows it to penetrate even acidic organelles in order to bind to DNA or RNA.

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.

Application :

Acridine orange base is used in a wide variety of biotechnology applications, including microscopy and flow cytometry.
It is commonly used for staining nucleic acids, including DNA and RNA, in cells and tissues. It intercalates into the nucleic acid strands and emits fluorescence, allowing for the visualization and analysis of DNA or RNA distribution, integrity, and cellular localization.DNA/RNA Quantification.
It is used to assess cell viability. It can distinguish between live cells (green fluorescence) and apoptotic or dead cells (orange-red fluorescence) based on their staining patterns.

Benefits :

Gives accurate, reliable, and reproducible results
Quantifying nucleic acids in solution
Stable at low pH

Additional information

Brand	Tristains
Size	10g, 5g

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TECHNICAL INFORMATION

Alternate Names	Acridine Orange base; 3,6-Bis(dimethylamino)acridine; Solvent Orange 15
Application	Acridine Orange Stain is a fluorescent nucleic acid binding dye which interacts with both DNA and RNA
CAS Number	494-38-2
Molecular Weight	265.35
Molecular Formula	C₁₇H₁₉N₃

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Refer to Certificate of Analysis for lot specific data (including water content).

Acridine Orange Stain is a fluorescent nucleic acid binding dye which interacts with both DNA and RNA. When bound to DNA, it emits green fluorescence (Em=525 nm) and when bound to RNA, it emits red fluorescence (Em=~650 nm).

TECHNICAL INFORMATION

Physical State	Solid
Solubility	Soluble in water (partly ), and 1 N HCl (1 mg/mL).
Storage	Store at room temperature
Melting Point	165° C (dec.)(lit.)
Boiling Point	468.58° C
Density	1.17 g/cm³
Refractive Index	n²⁰_D 1.71
IC50	Amyloid beta 42 fibril formation IC50 = 600 nM; R3230AC IC50 = 12.2 µM (human)
pK Values	pK_b: 9.97 (Predicted)

SAFETY INFORMATION

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

WGK Germany	3
RTECS	AR7600000
PubChem CID	62344
MDL Number	MFCD00005029
EC Number	200-614-0
Beilstein Registry	0020916
SMILES	CN(C)C1=CC2=C(C= C1)C=C3C=CC(=CC3=N2)N(C)C

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S10008

Acridine Orange Base

CAS No

494-38-2

Synonym(s)

3,6-Bis(dimethylamino)acridine; Solvent Orange 15

Molecular Formula:

C₁₇H₁₉N₃

Molecular Weight:

265.35

EC Number:

–

Colour Index Number:

46005

PubChem CID:

62344

MDL number:

MFCD00005029

Specification SDS

Brand	Tristains
Size	10g, 5g

Alternate Names	Acridine Orange base; 3,6-Bis(dimethylamino)acridine; Solvent Orange 15
Application	Acridine Orange Stain is a fluorescent nucleic acid binding dye which interacts with both DNA and RNA
CAS Number	494-38-2
Molecular Weight	265.35
Molecular Formula	C₁₇H₁₉N₃

Physical State	Solid
Solubility	Soluble in water (partly ), and 1 N HCl (1 mg/mL).
Storage	Store at room temperature
Melting Point	165° C (dec.)(lit.)
Boiling Point	468.58° C
Density	1.17 g/cm³
Refractive Index	n²⁰_D 1.71
IC50	Amyloid beta 42 fibril formation IC50 = 600 nM; R3230AC IC50 = 12.2 µM (human)
pK Values	pK_b: 9.97 (Predicted)

WGK Germany	3
RTECS	AR7600000
PubChem CID	62344
MDL Number	MFCD00005029
EC Number	200-614-0
Beilstein Registry	0020916
SMILES	CN(C)C1=CC2=C(C= C1)C=C3C=CC(=CC3=N2)N(C)C