Buy Acridine Orange Base (494-38-2) MF: C17H19N3 | MW: 265.35. Get High Quality Acridine Orange Base (494-38-2) from TriStains.
Acridine Orange Base a fluorescent dye, finds various applications in biological and biomedical research. Acridine Orange Base belongs to the class of aminoacridines, characterized by acridine with dimethylamino substituents at positions 3 and 6. Its hydrochloride salt forms the fluorescent dye ‘acridine orange,’ used for cell cycle determination, also known as Solvent Orange 15. Acridine Orange Base stability at low pH enables it to penetrate acidic organelles, binding to DNA or RNA.
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Application :
- It is used in a wide variety of biotechnology applications, including microscopy and flow cytometry.
- Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.
- Acridine Orange Base is used to assess cell viability and cytotoxicity. It can distinguish between live cells (green fluorescence) and apoptotic or dead cells (orange-red fluorescence) based on their staining patterns.
- Acridine Orange Base is commonly used for staining nucleic acids, including DNA and RNA, in cells and tissues. It intercalates into the nucleic acid strands and emits fluorescence, allowing for the visualization and analysis of DNA or RNA distribution, integrity, and cellular localization.DNA/RNA Quantification.
Benefits :
- Gives accurate, reliable, and reproducible results
- Quantifying nucleic acids in solution
- Its stable at low pH
- Used in cell cycle assays
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