Buy Acridine Orange (10127-02-3) MF: C17H19ClN3•HCl•1/2ZnCl2 | MW: 369.96. Get High Quality Acridine Orange (10127-02-3) from TriStains.
Acridine Orange is a cell-permeant dye binding to nucleic acids, emitting green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. Acridine Orange exhibits a maximum excitation at 502 nm and a maximum emission at 525 nm when bound to DNA, while bound to RNA, it displays a maximum emission at 650 nm and a maximum excitation at 460 nm. Acridine Orange also a positive solvatochromic dye and used as a lysosomal dye. Other names include 3,6-Acridinediamine, Acridine Orange Base, Acridine Orange NO, Basic Orange 14, etc. Acridine Orange finds common use as a fluorescent stain in microbiology and histology.
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Application :
- Acridine orange is useful for cell-cycle studies.
- It may also be useful as a method for measuring apoptosis, and for detecting intracellular pH gradients and the measurement of proton-pump activity2. This procedure optimally stains cells for analysis by flow cytometry.
- Acridine orange has been widely accepted and used in many different areas, such as epifluorescence microscopy, and the assessment of sperm chromatin quality.
- Acridine orange staining has to be performed at an acidic pH to obtain the differential staining, which allows bacterial cells to stain orange and tissue components to stain yellow or green.
Benefits :
- Able to withstand low pH environments
- Differentiate various types of cells (i.e., bacterial cells and white blood cells)
- High quality and good effectiveness
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