Buy Bromophenol Blue 0.01% (62625-28-9) MF: | MW: . Get High Quality Bromophenol Blue 0.01% (62625-28-9) from Tristains.
Bromophenol Blue is a member of the sulfonephthalein pH indicator family. It is commonly used as a sodium salt, which enhances its solubility and stability in aqueous solutions. Bromophenol Blue exhibits a color transition from yellow (acidic pH) to blue (alkaline pH).
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Applications :
pH Determination: Bromophenol Blue 0.4% solution is widely used as a pH indicator in laboratory settings. It can be added to a solution to determine its pH by observing the resulting color change. The transition occurs in the pH range of approximately 3.0 to 4.6.
Agarose Gel Electrophoresis: Bromophenol Blue 0.01% solution is commonly added to agarose gels used in DNA and RNA gel electrophoresis. It serves as a tracking dye that migrates alongside the nucleic acid samples during electrophoresis. The visible color change of the dye provides a visual indication of the migration front and helps monitor the progress of the separation.
Cell Culture: Bromophenol Blue 0.01% solution is occasionally used in cell culture applications to monitor pH changes in culture media. It can be added to the media to provide a visual indication of pH changes over time. This can be particularly useful in assessing the health and growth of cells in culture.
Laboratory Demonstrations: Bromophenol Blue 0.01% solution is often used in educational laboratory demonstrations to illustrate pH concepts and acid-base chemistry. Its color change provides a clear visual representation of pH variations and is useful for teaching basic principles of chemical reactions.
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