Description

Neutral Red, 90% CAS: 553-24-2 | MF: C15H17ClN4 | MW: 288.78, Buy now Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains. All Tristains products are exclusively distributed by Dawn Scientific Inc.

Neutral Red 90% is a crystalline powder that ranges in color from black brown to dark green and produces a red solution when mixed with water. This particular product is 90% pure Neutral Red, with a chemical formula of C15H17ClN4. It is a protonated diaminophenazine dye commonly used for staining tissue sections and cell smears in histology and cytology. Additionally, it serves as a pH indicator, transitioning from red to yellow within the pH range of 6.8 to 8.0. With its role as a dye, acid-base indicator, and two-color indicator.

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.

Application :

Neutral red 90% is also recommended for use in viability assessment as it is selectively absorbed by lysosomes and other acidic organelles in cells.
It is used as a nuclear stain, counterstain in the Gram procedure, either alone, or as a component of the Twort stain (a fast green-neutral red mixture).
It is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes.
Neutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes.

Benefits :

Selectively stains viable cells, making it a reliable indicator of cell viability
Versatile and ease of use
Compatible and cost-effective

Additional information

Size	100g, 10g, 25g, 50g
CAS NO	553-24-2
CI No	50040
MW	288.77
Brand	Tristains

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TECHNICAL INFORMATION

Alternate Names	3-Amino-7-dimethylamino-2-methylphenazine hydrochloride; Basic Red 5; Toluylene red
Application	Neutral Red is a common histology stain used to determine cell viability.
CAS Number	553-24-2
Molecular Weight	288.78
Molecular Formula	C₁₅H₁₇ClN₄

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

Neutral Red is most commonly used for histological stain for lysosymes but has also been incorporated in investigation of viruses, as a pH indicator, determination of DNA with optical and electrochemical methods, evalutaion of synthetic and naturally derived biomaterials. Neutral red can be electrochemically polymerized and used as a redox mediator. The compound once taken up by cells can be used to acertain cell viability with a excitation maximum at 470 nm and emission maximum at 580nm.

TECHNICAL INFORMATION

Appearance	Powder
Physical State	Solid
Solubility	Soluble in water (50 mg/ml at 25° C), abs. alcohol (1.8%), and cellosolve (3.75%).
Storage	Store at 4° C
Melting Point	290° C (lit.)(dec.)
Boiling Point	497.3° C at 760 mmHg
Refractive Index	n²⁰_D 1.75 (Predicted)
IC50 :	Internal ribosome entry site (IRES): IC₅₀= 20 µM (human); Huh-7: IC₅₀= 69.26 µM (human); -Arrestin-1: IC₅₀= 8.88 µM (human); Cytoplasmic zinc-finger protein: IC₅₀= 13.00 µM (Caenorhabditis elegans); Apaf-1: IC₅₀= 34 µM (human)

SAFETY INFORMATION

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

WGK Germany	3
RTECS	SG1400000
PubChem CID	11105
Merck Index	14:6488
MDL Number	MFCD00012651
EC Number	209-035-8
Beilstein Registry	3918740
SMILES	CC1=CC2=NC3=C(C=C(C=C3)N(C)C)N=C2C=C1N.Cl