Description

Trypan Blue CAS: 72-57-1 | MF: C34H24N6O14S4•4Na | MW: 960.81, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains.

Trypan Blue is a black solid powder that is used in laboratories and research. It is also called Direct Blue 14 or Niagara Blue 3B with a molecular formula of C34H24N6O14S4•4Na. Trypan Blue is a crucial stain that specifically stains the dead cells, hence it is an important reagent in cell viability tests. Its chemical stability, ease of use, and accuracy have seen it be a choice dye in biological studies, cell biology and histology studies.

Applications

Applied in cell culture laboratories to distinguish between dead and live cells to make proper viability determinations.
Is a critical tissue and cell stain to be examined by the microscope.
Used in microbiology to determine the viability of bacteria and fungi.

Key features

Selective Cell Staining: Stains dead cells while leaving viable cells unstained, ensuring accurate experimental outcomes.
Consistent and Reliable: Gives repeatable findings in experiments, biological, histological and microbiological.
Flexible and Cost-Effective: Capable of use in various staining processes, can be used in cell culture, microbiology, and histology.

General Description

Trypan blue (CAS: 72-57-1) is a reliable live cell stain in cell laboratories, and allows the practical differentiation of viable and non-viable cells. It is the best choice as a reagent in routine cell biology, microbiology and histology assays due to its chemical stability, solubility, and selective staining characteristics.

Want to buy Trypan Blue? Trypan Blue provided by TriStains guarantees high purity, consistent results and reliable performance; therefore, it is one of the preferred stains in professional laboratories and research centers of the USA. TriStains can be used by laboratories seeking to purchase Trypan Blue or check the lowest cost in the USA to offer high-quality grade and certified reagents that can be used to conduct cell viability investigations, histology, cytology, and microbiology studies. Order Trypan Blue today and enhance the accuracy, consistency, and reliability of your qualitative chemical analysis.

About Brand

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.

Additional information

CAS No	72-57-1
Synonyms	Direct Blue 14, Dianil blue, Diaminine Blue
CI No	23850
MW	960.81
Brand	Tristains
Size	100g, 10g, 25g, 50g

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TECHNICAL INFORMATION

Alternate Names	Direct Blue 14, Dianil blue, Diaminine Blue
Application	Trypan Blue is a viability dye for quantifying live versus dead cells
CAS Number	72-57-1
Molecular Weight	960.81
Molecular Formula:	C₃₄H₂₄N₆O₁₄S₄•₄Na

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples.

TECHNICAL INFORMATION

Appearance	Powder
Physical State	Solid
Solubility	Soluble in water (10 mg/ml at 25° C), ethanol (0.6 mg/ml), and EGME (20 mg/ml).
Storage	Store at 4° C
Melting Point	>300° C (lit.)
IC50	SH-PTP2: IC50 = 3.9 µM (human); Protein-tyrosine phosphatase 1: IC50 = 7.4 µM (Saccharomyces cerevisiae); P2Y2: IC50 = 17.1 µM (human)
pK Values	pKa: -1.53 (Predicted), pKb: 2.39 (Predicted)

SAFETY INFORMATION

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

WGK Germany	3
RTECS	QJ6475000
PubChem CID	9562061
Merck Index	14: 9792
MDL Number	MFCD00003969
EC Number	200-786-7
Beilstein Registry	4360496
SMILES	CC1=C(C=CC(=C1)C2=CC(=C(C=C2)NN=C3C(=CC4=CC (=CC(=C4C3=O)N)S(=O)(=O)[O-])S(=O)(=O)[O-])C)NN=C5C (=CC6=CC(=CC(=C6C5=O)N)S(=O)(=O)[O-]) S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+]

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S10193

Trypan Blue

CAS No

72-57-1

Synonym(s)

Direct Blue 14, Dianil blue, Diaminine Blue

Molecular Formula:

C₃₄H₂₄N₆Na₄O₁₄S₄

Molecular Weight:

960.81

EC Number:

–

Colour Index Number:

23850

PubChem CID:

6296

MDL number:

MFCD00003969

Specification SDS Available pack size: 100g, 10g, 25g, 50g

CAS No	72-57-1
Synonyms	Direct Blue 14, Dianil blue, Diaminine Blue
CI No	23850
MW	960.81
Brand	Tristains
Size	100g, 10g, 25g, 50g

Alternate Names	Direct Blue 14, Dianil blue, Diaminine Blue
Application	Trypan Blue is a viability dye for quantifying live versus dead cells
CAS Number	72-57-1
Molecular Weight	960.81
Molecular Formula:	C₃₄H₂₄N₆O₁₄S₄•₄Na

Appearance	Powder
Physical State	Solid
Solubility	Soluble in water (10 mg/ml at 25° C), ethanol (0.6 mg/ml), and EGME (20 mg/ml).
Storage	Store at 4° C
Melting Point	>300° C (lit.)
IC50	SH-PTP2: IC50 = 3.9 µM (human); Protein-tyrosine phosphatase 1: IC50 = 7.4 µM (Saccharomyces cerevisiae); P2Y2: IC50 = 17.1 µM (human)
pK Values	pKa: -1.53 (Predicted), pKb: 2.39 (Predicted)

WGK Germany	3
RTECS	QJ6475000
PubChem CID	9562061
Merck Index	14: 9792
MDL Number	MFCD00003969
EC Number	200-786-7
Beilstein Registry	4360496
SMILES	CC1=C(C=CC(=C1)C2=CC(=C(C=C2)NN=C3C(=CC4=CC (=CC(=C4C3=O)N)S(=O)(=O)[O-])S(=O)(=O)[O-])C)NN=C5C (=CC6=CC(=CC(=C6C5=O)N)S(=O)(=O)[O-]) S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+]