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Acridine Orange
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Tristains

S10007

Acridine Orange

CAS No
10127-02-3
Synonym(s)
3,6-Acridinediamine, Acridine Orange Base, Acridine Orange NO, Basic Orange 14
Molecular Formula:
C17H19ClN3•HCl•1/2ZnCl2
Molecular Weight:
369.96
EC Number:
233-353-6
Colour Index Number:
46005
PubChem CID:
159263
MDL number:
MFCD00081043
Specification SDS Available pack size: 10g, 25g
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Description

Acridine Orange| CAS: 10127-02-3 | MF: C17H19ClN3•HCl•½ZnCl2 | MW: 369.96 | Buy now for reliable and Reproducible results from TriStains.

Acridine Orange is an orange-brown to reddish-brown fluorescent powdered stain commonly used in laboratory stain techniques. It is a cell-permeant nucleic acid–binding dye that emits green fluorescence when bound to double-stranded DNA (dsDNA) and red fluorescence when bound to single-stranded DNA (ssDNA) or RNA. When bound to DNA, Acridine Orange shows a maximum excitation at 502 nm and maximum emission at 525 nm. It has a maximum excitation of 460 nm and emission of 650 nm when reacting with RNA. This dye can also be referred to as a positive solvatochromic dye and can be used as a lysosomal stain. The other synonyms are 3,6-Acridinediamine, Acridine Orange Base, Acridine Orange NO and Basic Orange 14.

Applications

  • Cell-Cycle Studies: It has the capacity to stain nucleic acid which can be used to study the cell-cycle and differentiate between different cell populations.
  • Bacterial Detection: Extensively used in microbiology in fluorescent bacteria detection in blood cultures and biological fluids.
  • Sterile Specimen Screening:Used for rapid screening of specimens that are normally sterile.
  • Genetics & Molecular Biology: Enables the detection of RNA and DNA, and assists genetic and molecular biology research.

Key features

  • pH Sensitive: Works well in low-pH, thus being applicable in various biological applications.
  • Cell Differentiation Support: Improves the distinction of the bacterial cells and the white blood cells with other cellular components.
  • High Quality & Reliable: Gives stable and powerful output in microscopy and fluorescence research.

General Description

Acridine Orange (CAS No. 10127-02-3) is an excellent quality fluorescent dye according to nucleic acid staining in histology, cytology, microbiology and biological research. It is an effective substitute in a laboratory due to its high fluorescence and cell permeability.

TriStains offers Acridine Orange that can be used in microscopy, bacteria identification, and molecular studies. Laboratories who want to receive quality with regular purchases will find this option of bulk purchasing. Do you need Acridine Orange for your laboratory or research purposes? Buy it from TriStains today.

About Brand

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.

Additional information
Brand

Tristains

Size

10g, 25g

CAS No

10127-02-3

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TECHNICAL INFORMATION
Alternate Names 3,6-Bis(dimethylamino)acridinium chloride hemi(zinc chloride salt)
Application Acridine Orange hemi(zinc chloride) salt is fluorescent dye that stains DNA and RNA
CAS Number 10127-02-3
Molecular Weight 369.96
Molecular Formula
C17H19ClN3•HCl•1/2ZnCl2

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Refer to Certificate of Analysis for lot specific data (including water content).

Acridine Orange hemi (zinc chloride) salt is a cell-permeable metachromatic fluorescent dye that stains DNA and RNA. This dye is also an inhibitor of RNA polymerase.

References
1. Wolfe, A.D. et al. 1972. Biochemistry. 11(9): 1569-1572. PMID: 4554949
2. Golden, J.F. and West, S.S. 1974. J. Histochem. Cytochem. 22(7): 495-505. PMID: 4136741
3. Golden, J.F. et al. 1979. J. Histochem. Cytochem. 27(1): 522-528. PMID: 374619
4. Frost, J.K. et al. 1979. J. Histochem. Cytochem. 27(1): 545-551. PMID: 86575
5. Tyrer, H.W. et al. 1979. J. Histochem. Cytochem. 27(1): 552-556. PMID: 86576
6. Paul, R.N. 1980. Stain Technol. 55(3): 195-196. PMID: 6161450

TECHNICAL INFORMATION

Appearance Powder
Physical State Solid
Solubility Soluble in water (1 mg/ml).
Storage Store at room temperature
Melting Point 165° C
Refractive Index
n20D ~1.71 (Predicted)
Fluoresence λex 502 nm, λem 525 nm; λex 460 nm, λem 650 nm

SAFETY INFORMATION

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany 3
RTECS AR7600000
PubChem CID 159263
MDL Number MFCD00081043
EC Number 233-353-6
Beilstein Registry 3734978
SMILES CN(C)C1=CC2=C(C=C1)C= C3C=CC(=CC3=N2)N(C)C.Cl.[Cl-].[Cl-].[Zn+2]

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