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Brilliant Blue G250
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Tristains

S10037

Brilliant Blue G250

CAS No
6104-58-1
Synonym(s)
Coomassie Brilliant Blue G (CBBG)
Molecular Formula:
C47H48N3NaO7S2
Molecular Weight:
854.02
EC Number:
228-058-4
Colour Index Number:
42655
PubChem CID:
6324599
MDL number:
MFCD00078482
Specification SDS Available pack size: 25g, 5g
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Description

Brilliant Blue G250|CAS: 6104-58-1|MF: C47H48N3O7S2·Na|MW: 854.02, Buy now for reliable and Reproducible results from TriStains.

Brilliant Blue G250 (Brilliant Blue G-250) is normally sold as a dark blue to purple powder. The dye is extensively used in molecular biology and biochemistry to selectively stain proteins. Brilliant Blue G250 (C.I. 42655) is a member of the Coomassie Brilliant Blue (CBB) family and a member of the triphenylmethane dye family. It has a C47H48N3NaO7S2 molecular formula. Other names used to identify the dye include Acid Blue 90, Brilliant Blue G, Cyanine G, Polar Blue G, SERVA BLUE G, and protein gel stain. Brilliant Blue G250 is highly affine to proteins and binds to them in solution selectively.

Applications

  • Brilliant Blue G250 is extensively used for protein visualization and separation in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), where proteins appear as blue bands against a clear background.
  • Coomassie Brilliant Blue G-250 is the dye of choice for the Bradford assay, a widely used method for quantitative protein determination.
  • Migration pattern of stained proteins on gels can be used to estimate the molecular weight since smaller proteins move faster compared to larger ones.

Key features

  • Sensitivity in identification of proteins in low concentrations.
  • Rapid and steady attachment to acidic and non-acidic proteins.
  • Staining to reproduce and be reliable in several protocols.
  • Excellent storage properties and good solubility in water and methanol.

General Description

Brilliant Blue G250, or Coomassie Brilliant Blue G-250 or Acid Blue 90, is a high-affinity synthetic stain that is commonly used to detect and quantify protein. Having a molecular formula of C47H48N3NaO7S2 and a Color Index number of C.I. 42655, this dye is a reliable reagent in molecular biology, biochemistry and proteomics laboratories

Within the TriStains range, the Brilliant Blue G250 has been supplied with a guarantee of the stability of the staining, consistent performance across batches and a reliable analysis outcome of the research laboratories and the academic institutions. Brilliant Blue G250 is priced depending on the purity grade, packaging size, and volume of the order. Customers can be contacted by the support services to give detailed quotation on technical specifications, availability, and bulk orders. Order Brilliant Blue G250 today at TriStains to find out the correct, reproducible and high-contrast results in protein staining.

About Brand

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.

Additional information
Brand

Tristains

Size

25g, 5g

CAS No

6104-58-1

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TECHNICAL INFORMATION
Alternate Names Coomassie Brilliant Blue G (CBBG)
Application Brilliant Blue G is a commonly used analytical biochemistry dye and P2X7 antagonist
CAS Number 6104-58-1
Molecular Weight 854.02
Molecular Formula: C47H48N3O7S2•Na

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

Brilliant Blue G (CBBG) is a member of the Coomassie dye family and is commonly used in analytical biochemistry to stain proteins in SDS-PAGE and BN-PAGE. CBBG is a useful component in the Bradford Method, a dye-binding protein assay employed to determine protein concentration in a given solution. CBBG can exist in three forms: a cation (red form with maximum absorbance at 470 nm), a neutral form (green with maximum at 650 nm), and an anionic form (blue with maximum at 595 nm). The anionic form initiates a stable complex with basic amino acid residues, mainly arginine and aromatic amino acids. Although the anionic form exists in negligible concentrations at the assay reaction mixture’s pH, the protein-binding shifts the equilibrium toward its formation. Recent research reveals CBBG as a P2X7 (P2X purinoceptor 7: P2RX7) antagonist and suggests that it exhibits neuroprotective properties in rat spinal cord cells.

TECHNICAL INFORMATION

Appearance Powder
Physical State Solid
Solubility Soluble in water (40 mg/ml at 20° C), methanol, ethanol (40 mg/ml), DMSO (~10 mg/ml), and DMF (~0.5 mg/ml).
Storage Store at room temperature
Melting Point >100° C

SAFETY INFORMATION

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany 3
RTECS 6324599
MDL Number MFCD00078482
EC Number 228-058-4
Beilstein Registry 5230822
SMILES CCN(CC1=CC(=CC=C1)S(=O)(=O)[O-])C2=CC(=C(C=C2)/C(=C/3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3C)/C5=CC=C(C=C5)NC6=CC=C(C=C6)OCC)C.[Na+]

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