Brilliant Blue G250|CAS: 6104-58-1|MF: C47H48N3O7S2·Na|MW: 854.02, Buy now for reliable and Reproducible results from TriStains.
Brilliant Blue G250 (Brilliant Blue G-250) is normally sold as a dark blue to purple powder. The dye is extensively used in molecular biology and biochemistry to selectively stain proteins. Brilliant Blue G250 (C.I. 42655) is a member of the Coomassie Brilliant Blue (CBB) family and a member of the triphenylmethane dye family. It has a C47H48N3NaO7S2 molecular formula. Other names used to identify the dye include Acid Blue 90, Brilliant Blue G, Cyanine G, Polar Blue G, SERVA BLUE G, and protein gel stain. Brilliant Blue G250 is highly affine to proteins and binds to them in solution selectively.
Applications
- Brilliant Blue G250 is extensively used for protein visualization and separation in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), where proteins appear as blue bands against a clear background.
- Coomassie Brilliant Blue G-250 is the dye of choice for the Bradford assay, a widely used method for quantitative protein determination.
- Migration pattern of stained proteins on gels can be used to estimate the molecular weight since smaller proteins move faster compared to larger ones.
Key features
- Sensitivity in identification of proteins in low concentrations.
- Rapid and steady attachment to acidic and non-acidic proteins.
- Staining to reproduce and be reliable in several protocols.
- Excellent storage properties and good solubility in water and methanol.
General Description
Brilliant Blue G250, or Coomassie Brilliant Blue G-250 or Acid Blue 90, is a high-affinity synthetic stain that is commonly used to detect and quantify protein. Having a molecular formula of C47H48N3NaO7S2 and a Color Index number of C.I. 42655, this dye is a reliable reagent in molecular biology, biochemistry and proteomics laboratories
Within the TriStains range, the Brilliant Blue G250 has been supplied with a guarantee of the stability of the staining, consistent performance across batches and a reliable analysis outcome of the research laboratories and the academic institutions. Brilliant Blue G250 is priced depending on the purity grade, packaging size, and volume of the order. Customers can be contacted by the support services to give detailed quotation on technical specifications, availability, and bulk orders. Order Brilliant Blue G250 today at TriStains to find out the correct, reproducible and high-contrast results in protein staining.
About Brand
TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.

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