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Tristains

S10037

Brilliant Blue G250

$34.23 – $94.23

CAS No
6104-58-1
Synonym(s)
Coomassie Brilliant Blue G (CBBG)
Molecular Formula:
C47H48N3NaO7S2
Molecular Weight:
854.02
EC Number:
228-058-4
Colour Index Number:
42655
PubChem CID:
6324599
MDL number:
MFCD00078482
Specification SDS

Available Options:

Description

Buy Brilliant Blue G250 (6104-58-1) MF: C47H48N3O7S2•Na | MW: 854.02. Get High Quality Brilliant Blue G250 (6104-58-1) from Tristains.

Brilliant Blue G250 typically appears as a dark blue or purple powder. Brilliant Blue G250, also known as Coomassie Brilliant Blue G-250, is a synthetic dye commonly used in biochemistry and molecular biology for protein staining. Brilliant blue G 250 (C.I. 42655) is a member of Coomassie brilliant blue (CBB), which is a triphenyl methane dye. Its molecular formula is C47H48N3NaO7S2. Brilliant Blue G250 is also known as Coomassie Brilliant blue G 250 (C.I. 42655), Acid blue 90, Brilliant Blue G, Cyanine G, Polar Blue G, SERVA BLUE G, protein gel stain. The dye has a high affinity for proteins, allowing it to selectively bind to and stain proteins in solution.

“TriStains”, Histological Stains/Biological Stains that offer range of stains used in Histology, Cytology, Microbiology and Hematology laboratories. TriStains meet the highest quality standards and give excellent color performance of desired components of cells and tissue in life science laboratories. TriStains series products are carefully tested to ensure accurate, reliable, and reproducible results. Our products are available in different packaging sizes to allow you to get all types of stains & Indicators for your specific purposes from a single source.

In-addition Tristains also deals in numerous Laboratory Supplies, Chemicals, Equipment, Instruments, Reagents, Standard Solutions, Buffers, Histological Stains/Biological Stains & Indicators and many more, for more information please visit our website www.tristains.com or email to sales@tristains.com we will be happy to help you. All Tristains Products are exclusively distributed by Dawn Scientific Inc (https://dawnscientific.com)

Application :

  • Brilliant Blue G250 is widely used for the visualization of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It is commonly used in the Coomassie Blue staining method, where proteins are visualized as blue bands against a clear background.
  • CBB G-250 (greenish tinted blue) dye is preferably used in Bradford assay for the quantification of proteins.
  • The migration pattern of stained proteins in a gel can be used to estimate the size of proteins, as smaller proteins generally migrate faster than larger ones.

Benefits :

  • High sensitivity in detecting proteins
  • Gives accurate and reliable results
  • Cost-effective
Additional information
Weight N/A
Dimensions N/A
Brand

Size

25g, 5g
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TECHNICAL INFORMATION
Alternate Names Coomassie Brilliant Blue G (CBBG)
Application Brilliant Blue G is a commonly used analytical biochemistry dye and P2X7 antagonist
CAS Number 6104-58-1
Molecular Weight 854.02
Molecular Formula: C47H48N3O7S2•Na

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

Brilliant Blue G (CBBG) is a member of the Coomassie dye family and is commonly used in analytical biochemistry to stain proteins in SDS-PAGE and BN-PAGE. CBBG is a useful component in the Bradford Method, a dye-binding protein assay employed to determine protein concentration in a given solution. CBBG can exist in three forms: a cation (red form with maximum absorbance at 470 nm), a neutral form (green with maximum at 650 nm), and an anionic form (blue with maximum at 595 nm). The anionic form initiates a stable complex with basic amino acid residues, mainly arginine and aromatic amino acids. Although the anionic form exists in negligible concentrations at the assay reaction mixture’s pH, the protein-binding shifts the equilibrium toward its formation. Recent research reveals CBBG as a P2X7 (P2X purinoceptor 7: P2RX7) antagonist and suggests that it exhibits neuroprotective properties in rat spinal cord cells.

TECHNICAL INFORMATION

Appearance Powder
Physical State Solid
Solubility Soluble in water (40 mg/ml at 20° C), methanol, ethanol (40 mg/ml), DMSO (~10 mg/ml), and DMF (~0.5 mg/ml).
Storage Store at room temperature
Melting Point >100° C

SAFETY INFORMATION

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany 3
RTECS 6324599
MDL Number MFCD00078482
EC Number 228-058-4
Beilstein Registry 5230822
SMILES CCN(CC1=CC(=CC=C1)S(=O)(=O)[O-])C2=CC(=C(C=C2)/C(=C/3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3C)/C5=CC=C(C=C5)NC6=CC=C(C=C6)OCC)C.[Na+]

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