Acridine Orange HCL

Buy Acridine Orange HCL (65-61-2) MF: C17H19N3 · HCl | MW: 301.81. Get High Quality Acridine Orange HCL (65-61-2) from Tristains.

Acridine orange HCL is Cell permeable fluorescent nucleic acid binding dye. At low concentration it intercalates into DNA and precipitates RNA. However, at high concentration it denatures and precipitates both RNA and DNA. It goes into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in the acidic organelle giving a bright red fluorescence. It is also known as Rhoduline Orange. Acridine Orange hydrochloride produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm).

“TriStains”, Histological Stains/Biological Stains that offer range of stains used in Histology, Cytology, Microbiology and Hematology laboratories. TriStains meet the highest quality standards and give excellent color performance of desired components of cells and tissue in life science laboratories. TriStains series products are carefully tested to ensure accurate, reliable, and reproducible results. Our products are available in different packaging sizes to allow you to get all types of stains & Indicators for your specific purposes from a single source.

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Application :

• Acridine Orange hydrochloride can be used to detect autophagic lysosomes and nuclei with orange/red fluorescent and green fluorescent, respectively. 
• It has also been used for the staining of chromosomes. Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans.

Benefits :

• Affordable and high quality product
• Used as a pH indicator
• Highly effective fluorescent stain