Fluorescein Diacetate

Fluorescein Diacetate CAS: 596-09-8 | MF: C24H16O7 | MW: 416.38, Buy now for reliable and reproducible results from Tristains.

Fluorescein Diacetate (FDA), or 3,6-Diacetoxyfluoran or Di-O-acetylfluorescein, is a derivative of fluorescein whose molecular formula is C24H16O7. It is a non-fluorescent and colorless substance that is capable of penetrating intact cell membranes. It is then broken down by intracellular esterases to become fluorescein, a green fluorescent molecule, when it enters viable cells. This attribute renders Fluorescein Diacetate very useful as a microbiological and cell biology tool of selective labeling, quantification and viability of living cells.

Applications

• Cell Biology and Live-Cell Imaging: Fluorescein Diacetate is widely used in cell viability assays. While non-fluorescent outside the cell, it is enzymatically converted to fluorescent fluorescein within viable cells, allowing clear differentiation between live and non-viable cells.
• Bacterial Viability Testing and Microbiology: Applications and Uses to determine the activity of both metabolism and esterase in bacterial cells, to aid the microbial viability studies.
• Flow Cytometry and Automated High-Throughput Screening: Interoperable with fluorescence-based flow cytometry platforms and with automated screening platforms to do large-scale analysis.

Key features

• High Sensitivity: Sensible ions of esterase activity are detected (very low), allowing an accurate determination of cell viability.
• Broad Range of Applications: Applicable to microorganisms, fungi, plant cells and mammalian cells.
• Stable: Gives predictable and repeatable outcomes in repeated experiments having long shelf life.
• Economical: Economical when using large-scale studies and repeated assays.

General Description

Fluorescein Diacetate, also known as Di-O-acetylfluorescein (CAS No. 596-09-8), is a non-fluorescent compound extensively used in cell biology, microbiology, plant science, and diagnostic laboratories. It is highly pure and has a great sensitivity that makes it suitable for cytochemical staining and the detection of enzymatic activity. Having a molecular weight of 416.38 g/mol and a molecular formula of C24H16O7, Fluorescein Diacetate provides high accuracy and reliability in fluorescence-based studies and is important in biology studies today.

Want to buy Fluorescein Diacetate? TriStains offers high-quality fluorescent stains and reagents used in laboratory work in histology, cytology, microbiology, hematology as well as the biological research laboratories. Fluorescein Diacetate comes in various packs depending on the research and institutional needs. Pricing will be determined by the quantity and packaging format, whereby optional pricing will be given on the purchase of large volumes, and a discount will be offered. Get in touch with TriStains and ask to be quoted to receive steady and high-quality research results.

About Brand

TriStains provides a marketplace for histology and biological stains, which is comprehensive enough to encompass the peculiar requirements of laboratories specializing in Histology, Cytology, Microbiology, and Hematology. With a reputation for exceeding quality expectations, TriStains performance is outstanding which allows for resolution of cell and tissue components fundamental to life sciences to be clearly visualized. Each product under TriStains series is validated for accuracy, reliability and consistency. TriStains, which manufactures and markets stains and indicators in various packing, offers laboratories turn key solutions for all their staining and indicator needs, improving accuracy in every experiment.