Fluorescein Free Acid

CAS No

2321-07-5

Synonym(s)

Fluorescein (free acid)

Molecular Formula:

C₂₀H₁₂O₅

Molecular Weight:

332.31

EC Number:

219-031-8

Colour Index Number:

45350:1

PubChem CID:

16850

MDL number:

MFCD00005050

Specification SDS

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Fluorescein free acid is a vibrant yellow-green fluorescent organic compound and a xanthene dye. Its chemical formula is C20H12O5. The fluorescein (free acid) molecule can be divided into two parts: one containing the xanthene moiety, which acts as a fluorophore, and the other bearing the benzene moiety, which controls fluorescence. Fluorescein displays a striking bright green fluorescence when exposed to ultraviolet or blue light. This versatile and extensively used fluorescent dye has diverse applications in various scientific and industrial fields, particularly in biology.

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Application :

Fluorescein (free acid) has been used as a staining agent in retinal imaging. Fluorescein (free acid) is widely used as a fluorescent stain and trace for many living cells and tissue applications.
Fluorescein is used in cell biology and microscopy to label and track cells, proteins, and other biomolecules.
It is also suitable as a marker for the study of motility of bile canaliculi.
Fluorescein angiography helps investigate changes in retinal blood supply. Fluorescein also plays a role as a physiological tracer to characterize the salmon′s intestinal permeability (Schep et al., 1998), and for the fluorophotometric measurement of aqueous humor flow.
It is widely employed as a quantum yield standard.